Ultraviolet-Visible(UV-Vis) spectroscopy is a technique used to quantify the light that is absorbed and scattered by a sample (a quantity known as the extinction, which is defined as the sum of absorbed and scattered light). In its simplest form, a sample is placed between a light source and a photodetector, and the intensity of a beam of light is measured before and after passing through the sample. These measurements are compared at each wavelength to quantify the sample’s wavelength-dependent extinction spectrum. The data is typically plotted absorbance as a function of wavelength. Each spectrum is background corrected using a “blank” (a cuvette filled with only the dispersing medium) to guarantee that spectral features from the solvent are not included in the sample spectrum. Our UV-Vis analysis is performed with Thermo Scientific GENESYS 10S Series UV-Vis spectrophotometer loaded with a high-intensity xenon lamp and dual-beam optical geometry. It can collect spectra from 190-1100 nm. The xenon lamp provides strong illumination across the ultraviolet, visible, and near-infrared electromagnetic spectrum. It is recommended to have at least 1 mL of solution without any particles to collect the spectrum. The solution will be loaded into either a quartz or plastic cell with a path length of 1 cm. The measurements of the samples as well as the solvent that is used to prepare the sample will be measured with the same instrumental configuration. The instrument has the following capabilities: Scan speeds up to 3,600nm/minute Have dual-beam optics for superior photometric accuracy Capable to acquire data from the UV to the near-IR Increase sample throughput with the integrated 6-cell changer Thermostating options with both circulating water and Peltier cooling (At the moment we don’t have the necessary accessories) Measure unusual or challenging samples with a variety of optional holders for test tubes, long path cuvettes, and filters

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UV-Visible Spectrophotometer

University of Sri Jayewardenepura

Product Category/ Test Name (Matrix):

UV-Visible Spectroscopy

Sub Category:

Optical Absorption of Transparent liquids, solids, thin-films and glass



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Manufacturer Thermo Scientific Funding Source
Model GENESYS 10S Series Whether the equipment service can be provided to external researchers/institutions Yes
Manufactured Year 0000 Availability of Technically Competent Staff
HS Code Instrument Condition Not Functioning
Vendor Name Commencement of Operations Date 0000-00-00
Vendor Contact InstrumentPrice 0.00
Vendor Url Stage of the Record Incomplete
Catalog Aceess
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Institute University of Sri Jayewardenepura
Faculty Faculty of Applied Science
Department Office of the Dean
Laboratory Instrument Centre
Contact Person Name Dr. Anushka Rajapaksha
Email anurajapaksha@sjp.ac.lk
Phone Number
Mobile Number 0774125340
Product Category UV-Visible Spectroscopy
Sub Category Optical Absorption of Transparent liquids, solids, thin-films and glass
Testing Paremeters light absorbed, transmitted, reflected by the sample, Concentration, Absorbing Wavelength
Description UV/VIS spectroscopy implies absorption/reflection measurements carried out in the ultraviolet and visible light spectrum. According to the Beer-Lambert law, which links the attenuation of light to the characteristics of the material through which the light passes, a sample’s absorbance is directly proportional to the concentration of the absorbing specimen. This technique is extensively used in research and analytical laboratories for the quantitative and qualitative analysis of all molecules and specific compounds that absorb ultraviolet and visible electromagnetic radiation. UV-Visible technique can be employed in impurity detection of compounds, concentration measurements, structure elucidation of organic compounds, studying chemical kinetics, and detection of functional groups etc.
Relevant Instruments UV-Visible Spectrometer
Technical Specification
Number of samples per cycle 0
Number of samples per day 0
Total usage hour per day 0

Under Development Stage

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