PDS-1000/He™ System

The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.

The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells

Key Features

    • A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
    • Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
    • Maximum control
    • High reproducibility

PDS-1000/He System with Hepta™ Adaptor

The Hepta adaptor for the PDS-1000/He biolistic system enables 7–10 times more cells to be transformed than the standard system. It fits into the shocking chamber of the PDS-1000/He system where it splits the helium shock wave over seven macrocarriers. By uniformly spreading the DNA-coated gold or tungsten particles over this larger area, the system maximizes the number of cells transformed during one bombardment. As the helium is split seven ways, pressure and particle velocities are reduced, making the system ideal for plants and cell cultures requiring less forceful penetration.

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Biolistic Particle Delivery Systems - Gene gun

University of Peradeniya

Product Category/ Test Name (Matrix):

Molecular Biology and Biotechnology

Sub Category:


Service Charge (Rs) -

Manufacturer Biorad Funding Source Asian Development Bank
Model PDS-1000 | He™ and Hepta™ Systems Whether the equipment service can be provided to external researchers/institutions Yes
Manufactured Year 0000 Availability of Technically Competent Staff Yes
HS Code Instrument Condition Partly malfunctioning
Vendor Name Commencement of Operations Date
Vendor Contact InstrumentPrice 0.00
Vendor Url Stage of the Record Completed
Catalog Aceess
Catalog Upload View File
Catalog Link
Institute University of Peradeniya
Faculty Agriculture
Department Agricultural Biotechnology Centre
Laboratory Agricultural Biotechnology Centre
Contact Person Name Prof. Pradeepa Bandaranayake
Phone Number 0812387180
Mobile Number
Product Category Molecular Biology and Biotechnology
Sub Category
Testing Paremeters PCR amplification, Melt Curve analysis
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Technical Specification



Aluminum, ABS plastic, and acrylic chassis

Weight    15 kg

Electrical input voltage     100–120 VAC, 50–60 Hz

Maximum current   <5 A




Fuse   6.3 A, 250 V, 5 x 20 mm

Vacuum   <0.4 inches mercury/min leakage

Overpressure     0.5 psi relief valve, self-resetting





Temperature: 0–35°C (32–95°F)
Humidity: 0–95% noncondensing


Temperature: 0–70°C (32–158°F)
Humidity: 0–95% noncondensing

Number of samples per cycle 0
Number of samples per day 0
Total usage hour per day 0

Under Development Stage

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